Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4539911..4540408 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5R25_RS21945 Protein ID WP_001173343.1
Coordinates 4539911..4540084 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5R25_RS21950 Protein ID WP_000398619.1
Coordinates 4540112..4540408 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS21935 (4536581) 4536581..4538038 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
M5R25_RS21940 (4538275) 4538275..4539792 + 1518 WP_022646456.1 lysine--tRNA ligase -
M5R25_RS21945 (4539911) 4539911..4540084 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5R25_RS21950 (4540112) 4540112..4540408 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5R25_RS21955 (4540637) 4540637..4540909 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5R25_RS21960 (4540921) 4540921..4541151 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
M5R25_RS21965 (4541332) 4541332..4542963 + 1632 WP_022646455.1 sensor histidine kinase -
M5R25_RS21970 (4542960) 4542960..4543679 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4531057..4540408 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256059 WP_001173343.1 NZ_CP103657:c4540084-4539911 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256059 WP_000398619.1 NZ_CP103657:c4540408-4540112 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References