Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4325800..4326376 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5R25_RS20870 Protein ID WP_001297643.1
Coordinates 4326089..4326376 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5R25_RS20865 Protein ID WP_000063156.1
Coordinates 4325800..4326102 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS20850 (4322396) 4322396..4324546 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5R25_RS20855 (4324596) 4324596..4324799 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5R25_RS20860 (4324810) 4324810..4325766 + 957 WP_001297640.1 GTPase -
M5R25_RS20865 (4325800) 4325800..4326102 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5R25_RS20870 (4326089) 4326089..4326376 - 288 WP_001297643.1 BrnT family toxin Toxin
M5R25_RS20875 (4326575) 4326575..4327272 - 698 Protein_4089 IS1 family transposase -
M5R25_RS20880 (4327329) 4327329..4327424 + 96 Protein_4090 dienelactone hydrolase family protein -
M5R25_RS20885 (4327570) 4327570..4328802 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M5R25_RS20890 (4328843) 4328843..4330123 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T256057 WP_001297643.1 NZ_CP103657:c4326376-4326089 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT256057 WP_000063156.1 NZ_CP103657:c4326102-4325800 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References