Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3042620..3043404 Replicon chromosome
Accession NZ_CP103657
Organism Escherichia coli strain 3150

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5R25_RS14625 Protein ID WP_000613626.1
Coordinates 3042910..3043404 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag M5R25_RS14620 Protein ID WP_024946541.1
Coordinates 3042620..3042913 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R25_RS14610 (3037781) 3037781..3038740 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5R25_RS14615 (3039313) 3039313..3042486 + 3174 WP_022645526.1 ribonuclease E -
M5R25_RS14620 (3042620) 3042620..3042913 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
M5R25_RS14625 (3042910) 3042910..3043404 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5R25_RS14630 (3043499) 3043499..3044452 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
M5R25_RS14635 (3044464) 3044464..3046107 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
M5R25_RS14640 (3046173) 3046173..3047114 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5R25_RS14645 (3047114) 3047114..3048211 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T256051 WP_000613626.1 NZ_CP103657:3042910-3043404 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT256051 WP_024946541.1 NZ_CP103657:3042620-3042913 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References