Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4454717..4455214 Replicon chromosome
Accession NZ_CP103645
Organism Escherichia coli strain 961

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5R18_RS21635 Protein ID WP_001173343.1
Coordinates 4454717..4454890 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5R18_RS21640 Protein ID WP_000398619.1
Coordinates 4454918..4455214 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R18_RS21625 (4451387) 4451387..4452844 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
M5R18_RS21630 (4453081) 4453081..4454598 + 1518 WP_001295074.1 lysine--tRNA ligase -
M5R18_RS21635 (4454717) 4454717..4454890 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5R18_RS21640 (4454918) 4454918..4455214 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5R18_RS21645 (4455441) 4455441..4455713 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
M5R18_RS21650 (4455725) 4455725..4455955 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
M5R18_RS21655 (4456136) 4456136..4457767 + 1632 WP_001216477.1 sensor histidine kinase -
M5R18_RS21660 (4457764) 4457764..4458483 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T256014 WP_001173343.1 NZ_CP103645:c4454890-4454717 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT256014 WP_000398619.1 NZ_CP103645:c4455214-4454918 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References