Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4157822..4158398 Replicon chromosome
Accession NZ_CP103645
Organism Escherichia coli strain 961

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5R18_RS20085 Protein ID WP_001297643.1
Coordinates 4158111..4158398 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag M5R18_RS20080 Protein ID WP_000063160.1
Coordinates 4157822..4158124 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R18_RS20065 (4154418) 4154418..4156568 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5R18_RS20070 (4156618) 4156618..4156821 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5R18_RS20075 (4156832) 4156832..4157788 + 957 WP_001297640.1 GTPase -
M5R18_RS20080 (4157822) 4157822..4158124 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
M5R18_RS20085 (4158111) 4158111..4158398 - 288 WP_001297643.1 BrnT family toxin Toxin
M5R18_RS20090 (4158597) 4158597..4159293 - 697 Protein_3924 IS1 family transposase -
M5R18_RS20095 (4159608) 4159608..4160840 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
M5R18_RS20100 (4160881) 4160881..4162161 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4158597..4158806 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T256011 WP_001297643.1 NZ_CP103645:c4158398-4158111 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT256011 WP_000063160.1 NZ_CP103645:c4158124-4157822 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References