Detailed information of TA system TA025599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2038479..2038923
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ACIFE_RS16365
• Protein ID: WP_202796614.1
• Coordinates: 2038738..2038923 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: G0JMC9
• Locus tag: ACIFE_RS09880
• Protein ID: WP_014029431.1
• Coordinates: 2038479..2038748 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS09860	2034206..2036746	+	2541	WP_014029426.1	DNA mismatch repair protein MutS	-
ACIFE_RS09865	2036948..2037478	+	531	WP_014029428.1	DedA family protein	-
ACIFE_RS09870	2037538..2037732	+	195	WP_014029429.1	hypothetical protein	-
ACIFE_RS09875	2037734..2038435	+	702	WP_049786850.1	hypothetical protein	-
ACIFE_RS09880	2038479..2038748	-	270	WP_014029431.1	hypothetical protein	Antitoxin
ACIFE_RS16365	2038738..2038923	-	186	WP_202796614.1	BrnT family toxin	Toxin
ACIFE_RS09885	2038985..2039209	+	225	WP_014029432.1	hypothetical protein	-
ACIFE_RS09890	2039272..2039541	+	270	WP_014029433.1	hypothetical protein	-
ACIFE_RS09895	2039538..2040461	-	924	WP_014029434.1	LysR family transcriptional regulator	-
ACIFE_RS09900	2040618..2042111	+	1494	WP_143217931.1	DHA2 family efflux MFS transporter permease subunit	-
ACIFE_RS09905	2042111..2043544	+	1434	WP_014029436.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (15-55)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6967.93 Da
• Isoelectric Point: 8.9727

>T25599 WP_202796614.1 NC_015942:c2038923-2038738 [Acidithiobacillus ferrivorans SS3]
VRYPESCGHIREVLIHGEPRYLVIGSINGKHWSAVVTYRGADIRLISARRSRTEEVALYES

Nucleotide

• Length: 186 bp

>T25599 NC_015942:c2038923-2038738 [Acidithiobacillus ferrivorans SS3]
GTGAGGTACCCTGAATCTTGTGGACACATTCGCGAAGTGCTGATTCACGGCGAGCCCCGATATCTGGTGATAGGATCTAT
CAACGGGAAACACTGGTCAGCTGTCGTCACCTACAGGGGTGCAGATATTCGACTTATTTCTGCTCGTCGATCACGAACCG
AGGAGGTAGCACTATATGAAAGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10360.89 Da
• Isoelectric Point: 10.3066

>AT25599 WP_014029431.1 NC_015942:c2038748-2038479 [Acidithiobacillus ferrivorans SS3]
MKAKTFDKQFDDGVDIIAALELSKAKRVLQEQKRVNVDFPTWMIESLDREARKLGITRQSIIKVWLAERLEESASNTTFQ
RTHRDAALR

Nucleotide

• Length: 270 bp

>AT25599 NC_015942:c2038748-2038479 [Acidithiobacillus ferrivorans SS3]
ATGAAAGCTAAAACGTTCGATAAACAGTTTGATGATGGTGTTGACATTATTGCTGCGCTGGAGTTATCCAAGGCGAAACG
CGTGTTGCAGGAGCAAAAGCGCGTTAATGTCGATTTTCCTACGTGGATGATTGAATCATTAGATCGGGAAGCAAGAAAGC
TCGGCATTACCCGGCAATCCATTATTAAGGTGTGGCTCGCTGAACGCCTTGAAGAGTCTGCTTCTAACACTACGTTCCAG
CGGACGCACCGCGATGCGGCGCTCCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E7XQ24