Detailed information of TA system TA255980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11119..11762
• Replicon: plasmid pMB5971_1
• Accession: NZ_CP103640
• Organism: Klebsiella aerogenes strain 722

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: M5T48_RS25320
• Protein ID: WP_016236302.1
• Coordinates: 11346..11762 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: M5T48_RS25315
• Protein ID: WP_001261282.1
• Coordinates: 11119..11349 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T48_RS25290 (M5T48_25290)	6835..7092	-	258	WP_023292103.1	hypothetical protein	-
M5T48_RS25295 (M5T48_25295)	7570..8298	+	729	Protein_6	CSS-motif domain-containing protein	-
M5T48_RS25300 (M5T48_25300)	8314..9150	+	837	WP_032439674.1	EAL domain-containing protein	-
M5T48_RS25305 (M5T48_25305)	9768..10199	-	432	WP_174805835.1	hypothetical protein	-
M5T48_RS25310 (M5T48_25310)	10740..11162	-	423	WP_046624301.1	hypothetical protein	-
M5T48_RS25315 (M5T48_25315)	11119..11349	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
M5T48_RS25320 (M5T48_25320)	11346..11762	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
M5T48_RS25325 (M5T48_25325)	11836..13398	+	1563	WP_004206609.1	AAA family ATPase	-
M5T48_RS25330 (M5T48_25330)	13383..14405	+	1023	WP_032439672.1	helicase UvrD	-
M5T48_RS25335 (M5T48_25335)	14950..15858	+	909	WP_032439686.1	HNH endonuclease	-
M5T48_RS25340 (M5T48_25340)	16044..16394	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / qnrB1 / aac(3)-IIa	-	1..170849	170849

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T255980 WP_016236302.1 NZ_CP103640:11346-11762 [Klebsiella aerogenes]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T255980 NZ_CP103640:11346-11762 [Klebsiella aerogenes]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT255980 WP_001261282.1 NZ_CP103640:11119-11349 [Klebsiella aerogenes]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT255980 NZ_CP103640:11119-11349 [Klebsiella aerogenes]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3