Detailed information of TA system TA025598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 1911276..1911895
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ACIFE_RS09230
• Protein ID: WP_202796659.1
• Coordinates: 1911276..1911626 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0JLJ8
• Locus tag: ACIFE_RS09235
• Protein ID: WP_014029300.1
• Coordinates: 1911626..1911895 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS09215	1906311..1909079	-	2769	WP_014029296.1	DUF3854 domain-containing protein	-
ACIFE_RS09220	1909841..1910065	-	225	WP_014029297.1	hypothetical protein	-
ACIFE_RS09225	1910239..1910685	+	447	WP_014029298.1	hypothetical protein	-
ACIFE_RS09230	1911276..1911626	-	351	WP_202796659.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACIFE_RS09235	1911626..1911895	-	270	WP_014029300.1	AbrB family transcriptional regulator	Antitoxin
ACIFE_RS09240	1912225..1912731	-	507	WP_014029301.1	hypothetical protein	-
ACIFE_RS09245	1912902..1913186	+	285	WP_014029302.1	hypothetical protein	-
ACIFE_RS09250	1913286..1913540	+	255	WP_014029303.1	hypothetical protein	-
ACIFE_RS09255	1913855..1914316	+	462	WP_014029305.1	hypothetical protein	-
ACIFE_RS09260	1914363..1915361	-	999	WP_014029306.1	phosphoadenosine phosphosulfate reductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1898720..1943762	45042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12675.32 Da
• Isoelectric Point: 7.9133

>T25598 WP_202796659.1 NC_015942:c1911626-1911276 [Acidithiobacillus ferrivorans SS3]
IGRKTPAPGDVYWINPNPTAGNEIRDRHRFVVITPTEINKLGLVTCVPITSGGNHARQNGLTVAISGHDTTGVAICNQMR
TFDLHARSGDVKYVETLDQQTTREIINRVTSIIDPA

Nucleotide

• Length: 351 bp

>T25598 NC_015942:c1911626-1911276 [Acidithiobacillus ferrivorans SS3]
ATCGGCCGGAAAACTCCAGCCCCAGGCGACGTCTACTGGATCAACCCCAACCCAACAGCAGGAAACGAAATCCGAGATCG
ACACCGGTTCGTGGTCATTACGCCGACCGAGATCAATAAACTTGGCCTGGTTACCTGCGTGCCTATCACCAGTGGCGGCA
ATCACGCCAGGCAAAACGGATTGACCGTGGCCATCTCTGGACACGACACGACGGGCGTCGCCATCTGCAATCAGATGCGT
ACCTTCGACCTGCACGCCAGATCTGGGGATGTGAAGTACGTGGAAACGCTGGACCAGCAAACCACACGCGAAATCATCAA
CCGGGTAACCAGTATCATCGATCCAGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9786.24 Da
• Isoelectric Point: 5.1316

>AT25598 WP_014029300.1 NC_015942:c1911895-1911626 [Acidithiobacillus ferrivorans SS3]
MLVTKIRKQGGAAVVTLPAEVLRQMDAGIGEVLNIEVSQHTITMRPVRGERRRYTLGELLAGVTEQDMQSLAEDTAWARE
GEPAGRELI

Nucleotide

• Length: 270 bp

>AT25598 NC_015942:c1911895-1911626 [Acidithiobacillus ferrivorans SS3]
ATGTTGGTCACAAAAATTCGCAAGCAAGGCGGTGCAGCCGTGGTAACACTGCCTGCAGAAGTCTTGAGGCAGATGGATGC
GGGGATCGGCGAGGTTTTGAATATTGAAGTATCTCAGCATACCATCACCATGCGCCCAGTACGCGGTGAGCGGCGGCGCT
ATACCCTGGGAGAATTACTCGCGGGTGTCACCGAGCAGGACATGCAGAGCCTCGCCGAGGACACCGCCTGGGCCAGAGAA
GGCGAGCCCGCCGGGAGAGAGTTGATTTGA

Structures

Antitoxin

• AlphaFold DB: G0JLJ8