Detailed information of TA system TA025597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1714259..1714905
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ACIFE_RS08265
• Protein ID: WP_202796656.1
• Coordinates: 1714519..1714905 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0JTK2
• Locus tag: ACIFE_RS08260
• Protein ID: WP_014029108.1
• Coordinates: 1714259..1714498 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS08230	1709282..1709902	-	621	WP_014029102.1	thioredoxin fold domain-containing protein	-
ACIFE_RS08235	1710322..1710567	+	246	WP_014029103.1	hypothetical protein	-
ACIFE_RS16775	1710781..1711016	+	236	Protein_1643	M48 family metallopeptidase	-
ACIFE_RS08245	1711936..1712505	+	570	WP_014029105.1	rusticyanin	-
ACIFE_RS08255	1713008..1714144	+	1137	WP_041650772.1	DNA polymerase IV	-
ACIFE_RS08260	1714259..1714498	+	240	WP_014029108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ACIFE_RS08265	1714519..1714905	+	387	WP_202796656.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIFE_RS16780	1715004..1715615	-	612	WP_014029110.1	hypothetical protein	-
ACIFE_RS17115	1715796..1715939	+	144	WP_014029111.1	hypothetical protein	-
ACIFE_RS08275	1716006..1716755	+	750	WP_014029112.1	PilZ domain-containing protein	-
ACIFE_RS08280	1717071..1717511	+	441	WP_014029113.1	VOC family protein	-
ACIFE_RS08285	1717654..1717941	+	288	WP_014029114.1	restriction endonuclease subunit S	-
ACIFE_RS08290	1717938..1718405	+	468	WP_014029115.1	tryptophan-rich sensory protein	-
ACIFE_RS08295	1718427..1718666	+	240	Protein_1654	pyridoxamine 5'-phosphate oxidase family protein	-
ACIFE_RS08300	1718680..1719237	+	558	WP_041649116.1	FUSC family protein	-
ACIFE_RS08305	1719298..1719879	+	582	WP_014029118.1	DUF4337 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• SpoVT-AbrB domain (10-55)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13721.88 Da
• Isoelectric Point: 6.6285

>T25597 WP_202796656.1 NC_015942:1714519-1714905 [Acidithiobacillus ferrivorans SS3]
MDTNILSDLIRHPHGIVAQRIAVVGEAAVCTSIVVACELRFGAAKKNSPRLTEQVNTIIAAMDVLPYDTPADQAYAEIRQ
YLAACGQPIGPNDLLIASHALAVKAILVSANVGEFSRVHGLIVHNWLQ

Nucleotide

• Length: 387 bp

>T25597 NC_015942:1714519-1714905 [Acidithiobacillus ferrivorans SS3]
CTGGACACCAACATTCTGTCGGATTTGATCAGGCATCCACATGGTATAGTGGCACAGAGGATTGCCGTTGTCGGCGAGGC
GGCCGTGTGCACCTCCATTGTGGTGGCTTGTGAATTGCGGTTTGGCGCAGCAAAGAAAAATTCTCCTCGACTCACAGAAC
AGGTGAACACCATTATCGCGGCCATGGATGTGCTCCCCTATGACACGCCGGCCGATCAGGCTTATGCGGAAATCCGTCAA
TACCTTGCCGCCTGCGGCCAGCCCATTGGTCCGAATGACTTGCTCATTGCCTCCCATGCACTGGCAGTCAAGGCCATATT
GGTGAGCGCCAATGTGGGCGAGTTTTCCCGAGTGCACGGGCTCATAGTGCACAATTGGCTACAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9332.60 Da
• Isoelectric Point: 4.7132

>AT25597 WP_014029108.1 NC_015942:1714259-1714498 [Acidithiobacillus ferrivorans SS3]
MHTERHVRFFRNGRNQAVRIPREFEMDAQEAIMRREDDRLIIEPVKRKGLLATLASLSALDEEFPDTDRDLLPLDDIEL

Nucleotide

• Length: 240 bp

>AT25597 NC_015942:1714259-1714498 [Acidithiobacillus ferrivorans SS3]
ATGCATACGGAACGCCATGTACGTTTTTTTCGTAATGGCCGCAACCAAGCGGTTCGTATTCCCCGTGAGTTCGAGATGGA
CGCGCAAGAGGCCATCATGCGGCGGGAGGATGACCGCTTGATCATCGAGCCCGTAAAACGCAAAGGGCTTCTCGCTACGT
TGGCGAGTCTTTCTGCCCTGGATGAGGAGTTCCCGGATACGGATCGCGATCTTCTCCCACTGGATGACATCGAATTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A060UNW3