Detailed information of TA system TA025595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1543165..1543645
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: ACIFE_RS07400
• Protein ID: WP_198661438.1
• Coordinates: 1543165..1543428 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: G0JS88
• Locus tag: ACIFE_RS07405
• Protein ID: WP_014028942.1
• Coordinates: 1543421..1543645 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS07360	1538611..1539546	-	936	WP_014028933.1	hypothetical protein	-
ACIFE_RS07365	1539909..1540322	-	414	WP_014028934.1	PIN domain nuclease	-
ACIFE_RS07370	1540309..1540524	-	216	WP_014028935.1	type II toxin-antitoxin system VapB family antitoxin	-
ACIFE_RS07375	1540700..1540927	-	228	WP_014028937.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
ACIFE_RS07380	1541083..1541877	-	795	WP_014028938.1	Fic family protein	-
ACIFE_RS16740	1541876..1542124	+	249	WP_049786842.1	hypothetical protein	-
ACIFE_RS07390	1542364..1542726	-	363	WP_190277971.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ACIFE_RS07395	1542687..1542944	-	258	WP_014028940.1	antitoxin	-
ACIFE_RS07400	1543165..1543428	-	264	WP_198661438.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACIFE_RS07405	1543421..1543645	-	225	WP_014028942.1	TraY domain-containing protein	Antitoxin
ACIFE_RS07410	1544234..1544524	-	291	WP_014028943.1	DUF1778 domain-containing protein	-
ACIFE_RS07415	1544652..1544930	-	279	WP_014028944.1	CopG family ribbon-helix-helix protein	-
ACIFE_RS07420	1544942..1545160	-	219	WP_014028945.1	DUF2283 domain-containing protein	-
ACIFE_RS07425	1545157..1545402	-	246	WP_014028946.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-85)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10031.78 Da
• Isoelectric Point: 10.4598

>T25595 WP_198661438.1 NC_015942:c1543428-1543165 [Acidithiobacillus ferrivorans SS3]
MIEIDDKVKKDLAALDKAIVKRITTFLRERIAQLDDPRSIGEALKGSKLGAFWKYRVGDYRIIASIEDSALRILVIRIGN
RKEVYRQ

Nucleotide

• Length: 264 bp

>T25595 NC_015942:c1543428-1543165 [Acidithiobacillus ferrivorans SS3]
ATGATTGAGATTGACGACAAGGTTAAGAAGGACTTGGCCGCGCTGGACAAGGCCATTGTCAAGCGCATCACGACGTTTCT
GCGCGAGCGTATTGCCCAGCTCGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGTTCCAAGCTGGGAGCCTTCTGGA
AGTACCGCGTTGGCGACTATCGAATCATTGCCAGCATCGAGGACAGTGCTTTGCGTATCCTCGTTATTCGCATCGGCAAT
CGCAAGGAGGTTTACCGTCAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8519.59 Da
• Isoelectric Point: 4.2910

>AT25595 WP_014028942.1 NC_015942:c1543645-1543421 [Acidithiobacillus ferrivorans SS3]
MLAIRLPAEVETRLEALAQATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSRTYTLDEVERDLGLDD

Nucleotide

• Length: 225 bp

>AT25595 NC_015942:c1543645-1543421 [Acidithiobacillus ferrivorans SS3]
ATGCTTGCCATCCGACTACCCGCCGAAGTGGAAACCCGCCTTGAAGCACTGGCGCAGGCTACCGGGCGCACCAAGACCTT
CTATGCCCGCGAAGCCATCCTTGAGCACTTGGACGACCTCGAAGATTTGTACCTTGCAGAGCAACGCCTGATCGACATTC
GAGCAGGACGCAGCCGAACGTACACGCTGGACGAAGTGGAGCGCGATCTTGGCTTGGATGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E7XSP6