Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3100659..3101443 Replicon chromosome
Accession NZ_CP103633
Organism Escherichia coli strain 2900

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5S90_RS15070 Protein ID WP_000613626.1
Coordinates 3100949..3101443 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M5S90_RS15065 Protein ID WP_001110447.1
Coordinates 3100659..3100952 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S90_RS15055 (3095809) 3095809..3096768 - 960 WP_000846343.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5S90_RS15060 (3097341) 3097341..3100526 + 3186 WP_001522867.1 ribonuclease E -
M5S90_RS15065 (3100659) 3100659..3100952 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M5S90_RS15070 (3100949) 3100949..3101443 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5S90_RS15075 (3101538) 3101538..3102491 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M5S90_RS15080 (3102503) 3102503..3104146 - 1644 WP_001522865.1 flagellar hook-associated protein FlgK -
M5S90_RS15085 (3104212) 3104212..3105153 - 942 WP_001522863.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5S90_RS15090 (3105153) 3105153..3106250 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T255937 WP_000613626.1 NZ_CP103633:3100949-3101443 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT255937 WP_001110447.1 NZ_CP103633:3100659-3100952 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References