Detailed information of TA system TA025593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1194953..1195545
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: ACIFE_RS05645
• Protein ID: WP_014028602.1
• Coordinates: 1194953..1195291 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: G0JPB5
• Locus tag: ACIFE_RS05650
• Protein ID: WP_014028603.1
• Coordinates: 1195291..1195545 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS05625	1191581..1192714	-	1134	WP_148260322.1	polysaccharide biosynthesis/export family protein	-
ACIFE_RS05630	1192686..1193714	-	1029	WP_014028598.1	phosphatase PAP2 family protein	-
ACIFE_RS05635	1193724..1193999	-	276	WP_014028599.1	hypothetical protein	-
ACIFE_RS05640	1193962..1194435	-	474	WP_014028600.1	clan AA aspartic protease	-
ACIFE_RS17080	1194719..1194886	-	168	WP_014028601.1	hypothetical protein	-
ACIFE_RS05645	1194953..1195291	-	339	WP_014028602.1	type II toxin-antitoxin system ChpB family toxin	Toxin
ACIFE_RS05650	1195291..1195545	-	255	WP_014028603.1	AbrB family transcriptional regulator	Antitoxin
ACIFE_RS05655	1195717..1196142	-	426	WP_014028604.1	type II toxin-antitoxin system VapC family toxin	-
ACIFE_RS05660	1196139..1196384	-	246	WP_014028605.1	prevent-host-death protein	-
ACIFE_RS05665	1196608..1196832	-	225	WP_014028606.1	hypothetical protein	-
ACIFE_RS05670	1197141..1197476	+	336	WP_014028608.1	transposase	-
ACIFE_RS05675	1197584..1199038	-	1455	WP_014028609.1	undecaprenyl-phosphate galactose phosphotransferase WbaP	-
ACIFE_RS17085	1198977..1199123	-	147	WP_014028610.1	hypothetical protein	-
ACIFE_RS05680	1199266..1200259	-	994	Protein_1135	UDP-glucose 4-epimerase GalE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

• Antitoxin PemI-like (4-70)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12193.02 Da
• Isoelectric Point: 9.2314

>T25593 WP_014028602.1 NC_015942:c1195291-1194953 [Acidithiobacillus ferrivorans SS3]
MRWPRRGEIWQADLNPTVGREQQGHRPMLVVSTEAFNRSGLVLVCPITQGGNQARFAGFAVSLMGSGTATQGVVMSNQSR
TVDLIARGGKFVEVTPDYVVSEVMAKMQAILE

Nucleotide

• Length: 339 bp

>T25593 NC_015942:c1195291-1194953 [Acidithiobacillus ferrivorans SS3]
ATGCGGTGGCCACGACGCGGGGAAATTTGGCAGGCAGACCTCAATCCAACTGTAGGCCGCGAACAACAAGGCCATCGCCC
TATGCTGGTAGTATCGACAGAAGCTTTCAATCGCTCAGGCCTGGTATTGGTTTGTCCTATCACCCAAGGCGGCAACCAGG
CCAGATTTGCGGGCTTTGCGGTCTCCTTGATGGGTTCTGGAACAGCCACACAGGGGGTGGTGATGAGCAACCAATCACGC
ACCGTCGACCTTATTGCCAGAGGCGGTAAGTTTGTCGAGGTGACGCCTGATTACGTGGTCAGCGAAGTGATGGCCAAGAT
GCAGGCCATTTTGGAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9311.55 Da
• Isoelectric Point: 4.7023

>AT25593 WP_014028603.1 NC_015942:c1195545-1195291 [Acidithiobacillus ferrivorans SS3]
MSQTKIQKWGHSMALRIPATTVRSWGVGEGQAVSLNIENGALVARPAQKRYSLEELLSQCDFTQPMSAEDREWLDAPPVG
IEDI

Nucleotide

• Length: 255 bp

>AT25593 NC_015942:c1195545-1195291 [Acidithiobacillus ferrivorans SS3]
ATGAGCCAGACCAAAATTCAGAAGTGGGGCCACAGCATGGCGCTACGCATACCCGCCACTACAGTGCGGAGCTGGGGCGT
CGGAGAAGGCCAGGCGGTATCATTGAACATTGAGAACGGGGCACTGGTGGCAAGGCCCGCACAAAAGCGTTACAGCCTGG
AGGAACTGCTGTCACAGTGCGATTTCACTCAACCTATGAGCGCGGAGGATCGTGAGTGGTTAGATGCACCACCTGTAGGC
ATTGAGGATATTTGA

Structures

Antitoxin

• AlphaFold DB: G0JPB5