Detailed information of TA system TA025592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1162285..1162922
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: ACIFE_RS05480
• Protein ID: WP_014028570.1
• Coordinates: 1162285..1162692 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: G0JP83
• Locus tag: ACIFE_RS05485
• Protein ID: WP_014028571.1
• Coordinates: 1162692..1162922 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS05440	1157780..1158094	-	315	WP_014028564.1	nucleotidyltransferase domain-containing protein	-
ACIFE_RS05445	1158087..1158479	-	393	WP_014028565.1	nucleotidyltransferase substrate binding protein	-
ACIFE_RS05450	1158645..1158977	-	333	WP_014028566.1	type II toxin-antitoxin system HicB family antitoxin	-
ACIFE_RS05455	1159211..1159411	+	201	Protein_1089	clan AA aspartic protease	-
ACIFE_RS05460	1159562..1160899	+	1338	WP_014028568.1	ISNCY-like element ISAcif1 family transposase	-
ACIFE_RS05465	1160903..1161811	+	909	WP_014028569.1	DUF4338 domain-containing protein	-
ACIFE_RS05470	1161891..1162073	+	183	WP_202796637.1	hypothetical protein	-
ACIFE_RS16200	1162096..1162239	+	144	Protein_1093	HU family DNA-binding protein	-
ACIFE_RS05480	1162285..1162692	-	408	WP_014028570.1	type II toxin-antitoxin system VapC family toxin	Toxin
ACIFE_RS05485	1162692..1162922	-	231	WP_014028571.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ACIFE_RS05490	1163463..1164650	+	1188	WP_202796638.1	ATP-binding protein	-
ACIFE_RS05495	1164672..1166114	-	1443	WP_014028573.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
ACIFE_RS05500	1166134..1166466	-	333	WP_014028574.1	DUF5615 family PIN-like protein	-
ACIFE_RS05505	1166463..1166687	-	225	WP_014028575.1	DUF433 domain-containing protein	-
ACIFE_RS05510	1166769..1167788	-	1020	WP_014028576.1	GDP-mannose 4,6-dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1159562..1160899	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15186.36 Da
• Isoelectric Point: 7.4207

>T25592 WP_014028570.1 NC_015942:c1162692-1162285 [Acidithiobacillus ferrivorans SS3]
MSQGYLLDTNICIYITKHHPPEVRARFAQHRAAELGMSVVTFGELIYGAEKSQARDKALKVLDQLRSSIQVLEMPAVAGQ
HYGQIRARLEKMGKPIGNNDLWIAAHALSEDRVLVTNNTQEFSRVEGLQLENWVS

Nucleotide

• Length: 408 bp

>T25592 NC_015942:c1162692-1162285 [Acidithiobacillus ferrivorans SS3]
ATGTCGCAGGGATACCTTCTGGACACTAATATCTGTATTTATATAACCAAGCATCATCCTCCTGAAGTGCGAGCACGTTT
TGCGCAACACCGGGCAGCCGAGTTGGGTATGTCGGTCGTGACATTCGGAGAACTGATTTACGGTGCGGAAAAAAGCCAGG
CGCGGGACAAAGCGCTAAAGGTCCTTGATCAGCTTCGATCCAGCATTCAGGTTCTGGAAATGCCAGCAGTTGCGGGTCAG
CACTATGGGCAAATCCGCGCAAGACTGGAAAAAATGGGAAAGCCTATCGGCAATAACGACCTCTGGATTGCCGCGCATGC
ACTGAGCGAGGATCGGGTACTGGTTACCAACAACACCCAGGAGTTTAGCCGGGTCGAGGGGTTGCAGCTGGAAAATTGGG
TGAGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8698.84 Da
• Isoelectric Point: 4.8011

>AT25592 WP_014028571.1 NC_015942:c1162922-1162692 [Acidithiobacillus ferrivorans SS3]
MTKARVFMSGNSQAVRLPKEFRFRTGEVDIFRRGDEIVLRERVGDASEIFDLLASLPDDFLAEGRQDTIPQQREGL

Nucleotide

• Length: 231 bp

>AT25592 NC_015942:c1162922-1162692 [Acidithiobacillus ferrivorans SS3]
ATGACCAAAGCGCGCGTATTCATGTCTGGGAATAGCCAAGCCGTCAGGCTGCCCAAGGAGTTTCGTTTTCGTACGGGGGA
GGTTGACATCTTCCGGCGTGGCGACGAGATAGTTTTGCGCGAGCGCGTGGGCGACGCGTCAGAGATTTTCGATCTGTTGG
CCAGCTTGCCCGATGATTTCTTGGCAGAGGGTCGGCAAGATACTATCCCGCAGCAACGCGAAGGGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1E7XRF4