Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 76708..77267 Replicon plasmid pMB6420_1
Accession NZ_CP103624
Organism Escherichia coli strain 3985

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag M5T36_RS23700 Protein ID WP_000421257.1
Coordinates 76992..77267 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y625
Locus tag M5T36_RS23695 Protein ID WP_001178087.1
Coordinates 76708..76992 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T36_RS23675 (M5T36_23675) 71897..72517 - 621 WP_000176497.1 DUF1449 family protein -
M5T36_RS23680 (M5T36_23680) 72784..73446 - 663 WP_000154142.1 hypothetical protein -
M5T36_RS23685 (M5T36_23685) 73587..74093 - 507 WP_001335626.1 transcription termination/antitermination NusG family protein -
M5T36_RS23690 (M5T36_23690) 74931..75809 + 879 WP_000729439.1 incFII family plasmid replication initiator RepA -
M5T36_RS23695 (M5T36_23695) 76708..76992 + 285 WP_001178087.1 ribbon-helix-helix domain-containing protein Antitoxin
M5T36_RS23700 (M5T36_23700) 76992..77267 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M5T36_RS23705 (M5T36_23705) 77373..77576 + 204 Protein_86 hypothetical protein -
M5T36_RS23710 (M5T36_23710) 77845..78111 - 267 WP_000627831.1 hypothetical protein -
M5T36_RS23715 (M5T36_23715) 78249..78851 - 603 WP_000517694.1 ProQ/FINO family protein -
M5T36_RS23720 (M5T36_23720) 79205..80551 + 1347 WP_001132037.1 protein YagA -
M5T36_RS23725 (M5T36_23725) 81062..81364 - 303 WP_001245884.1 hypothetical protein -
M5T36_RS23730 (M5T36_23730) 81361..81987 - 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) - 1..86833 86833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T255891 WP_000421257.1 NZ_CP103624:76992-77267 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10488.92 Da        Isoelectric Point: 5.6558

>AT255891 WP_001178087.1 NZ_CP103624:76708-76992 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB U9Y625

References