Detailed information of TA system TA025589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 696822..697369
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0JLC6
• Locus tag: ACIFE_RS03220
• Protein ID: WP_014028147.1
• Coordinates: 696822..697157 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ACIFE_RS03225
• Protein ID: WP_014028148.1
• Coordinates: 697154..697369 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS17030	691904..692065	+	162	WP_014028143.1	hypothetical protein	-
ACIFE_RS03205	692075..692296	+	222	WP_014028144.1	hypothetical protein	-
ACIFE_RS03210	692379..693389	+	1011	WP_014028145.1	virulence RhuM family protein	-
ACIFE_RS03215	693386..696622	+	3237	WP_014028146.1	type I restriction endonuclease subunit R	-
ACIFE_RS03220	696822..697157	-	336	WP_014028147.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ACIFE_RS03225	697154..697369	-	216	WP_014028148.1	antitoxin MazE family protein	Antitoxin
ACIFE_RS03230	697640..698146	-	507	WP_014028149.1	hypothetical protein	-
ACIFE_RS03235	698146..698478	-	333	WP_014028150.1	DUF3579 domain-containing protein	-
ACIFE_RS03240	698543..699439	-	897	WP_014028151.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-
ACIFE_RS03245	699519..700214	-	696	WP_014028152.1	DUF502 domain-containing protein	-
ACIFE_RS03250	700287..701672	-	1386	WP_041648472.1	sigma-54 dependent transcriptional regulator	-
ACIFE_RS03255	701674..702165	-	492	WP_014028154.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	669683..824386	154703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-106)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 11889.89 Da
• Isoelectric Point: 10.1007

>T25589 WP_014028147.1 NC_015942:c697157-696822 [Acidithiobacillus ferrivorans SS3]
VNDARRGDLLTVAIRGDFGKPRPALVVQSDQFSEQATVTVLLLSGTLVDAPLFRITVHPDGTNGLQKTSQIMVDKAMTVK
RDKLGPVFGSISAQVIIEVSRCLAVFLGIAK

Nucleotide

• Length: 336 bp

>T25589 NC_015942:c697157-696822 [Acidithiobacillus ferrivorans SS3]
GTGAACGACGCGCGGCGTGGTGATTTGTTGACGGTGGCTATACGGGGTGACTTCGGGAAACCGAGACCGGCGCTGGTGGT
GCAGTCTGATCAGTTTAGCGAGCAGGCGACCGTCACCGTGTTACTACTGTCGGGAACGCTGGTGGATGCGCCATTGTTCC
GGATAACAGTACATCCTGACGGGACCAATGGATTACAAAAGACTTCACAGATCATGGTCGATAAGGCAATGACGGTAAAG
CGCGATAAGTTGGGCCCCGTATTTGGTTCCATTAGTGCGCAGGTGATAATAGAGGTGAGTCGTTGCCTGGCGGTGTTTTT
AGGTATCGCAAAGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8252.26 Da
• Isoelectric Point: 5.0742

>AT25589 WP_014028148.1 NC_015942:c697369-697154 [Acidithiobacillus ferrivorans SS3]
MMTTHVNERVQKCRNALRRAGLRPVQIWVPDTRRPGFAEECHRQSRLAAQADRADTDLMEFLDDALDDLDE

Nucleotide

• Length: 216 bp

>AT25589 NC_015942:c697369-697154 [Acidithiobacillus ferrivorans SS3]
ATGATGACCACCCACGTGAATGAGCGCGTCCAAAAGTGCCGCAATGCTCTGCGCAGAGCTGGCCTCCGTCCAGTTCAAAT
CTGGGTGCCAGACACGCGCCGCCCCGGATTTGCAGAAGAGTGCCATCGCCAATCCCGATTAGCTGCCCAAGCAGATAGGG
CGGATACGGATCTGATGGAGTTTCTGGATGACGCACTGGATGATCTGGACGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1E7XTJ8