Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4349891..4350388 Replicon chromosome
Accession NZ_CP103623
Organism Escherichia coli strain 3985

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5T36_RS21025 Protein ID WP_001173343.1
Coordinates 4349891..4350064 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5T36_RS21030 Protein ID WP_000398619.1
Coordinates 4350092..4350388 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T36_RS21015 (4346561) 4346561..4348018 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
M5T36_RS21020 (4348255) 4348255..4349772 + 1518 WP_001295074.1 lysine--tRNA ligase -
M5T36_RS21025 (4349891) 4349891..4350064 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5T36_RS21030 (4350092) 4350092..4350388 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5T36_RS21035 (4350615) 4350615..4350887 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5T36_RS21040 (4350899) 4350899..4351129 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
M5T36_RS21045 (4351310) 4351310..4352941 + 1632 WP_001216487.1 sensor histidine kinase -
M5T36_RS21050 (4352938) 4352938..4353657 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island sul1 / qacE / ant(3'')-Ia / ant(2'')-Ia / floR / dfrA36 / sul2 - 4282722..4350388 67666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255889 WP_001173343.1 NZ_CP103623:c4350064-4349891 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT255889 WP_000398619.1 NZ_CP103623:c4350388-4350092 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References