Detailed information of TA system TA025588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 431965..432518
• Replicon: chromosome
• Accession: NC_015942
• Organism: Acidithiobacillus ferrivorans SS3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0JT96
• Locus tag: ACIFE_RS02040
• Protein ID: WP_014027921.1
• Coordinates: 432186..432518 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0JT95
• Locus tag: ACIFE_RS02035
• Protein ID: WP_014027920.1
• Coordinates: 431965..432186 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACIFE_RS02005	427118..427741	+	624	WP_014027914.1	DotI/IcmL/TraM family protein	-
ACIFE_RS02010	427754..428854	+	1101	WP_014027915.1	hypothetical protein	-
ACIFE_RS15775	428844..430070	+	1227	WP_014027916.1	DotG/IcmE/VirB10 family protein	-
ACIFE_RS02020	430081..430638	+	558	WP_014027917.1	sigma-70 family RNA polymerase sigma factor	-
ACIFE_RS02025	430719..431330	+	612	WP_014027918.1	hypothetical protein	-
ACIFE_RS02030	431346..431855	+	510	WP_014027919.1	hypothetical protein	-
ACIFE_RS02035	431965..432186	+	222	WP_014027920.1	DUF433 domain-containing protein	Antitoxin
ACIFE_RS02040	432186..432518	+	333	WP_014027921.1	DUF5615 family PIN-like protein	Toxin
ACIFE_RS02045	432967..433888	-	922	Protein_410	integron integrase	-
ACIFE_RS02050	434146..434547	+	402	WP_014027922.1	hypothetical protein	-
ACIFE_RS02055	434620..436137	+	1518	WP_014027923.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	283765..484935	201170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-76)

Antitoxin

• Protein of unknown function DUF433 (6-61)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12566.53 Da
• Isoelectric Point: 5.6753

>T25588 WP_014027921.1 NC_015942:432186-432518 [Acidithiobacillus ferrivorans SS3]
MQFLVDAQLPPALARWIRAQGYPARHVADIGMASALDPEIWDRAYGDGAIIITKDEDFATLRTIHPDGPTIIWLRTPNCR
RQILLDGFARLWPQMLDRLNQGEMLIELRS

Nucleotide

• Length: 333 bp

>T25588 NC_015942:432186-432518 [Acidithiobacillus ferrivorans SS3]
ATGCAGTTTTTAGTGGATGCGCAGCTACCACCCGCTCTGGCCCGATGGATCAGAGCGCAAGGTTATCCAGCCCGCCATGT
TGCCGACATAGGTATGGCCAGCGCTCTTGATCCGGAAATATGGGATCGTGCATATGGGGATGGCGCTATCATCATAACCA
AGGATGAAGATTTCGCTACACTCCGCACGATACATCCCGATGGCCCCACTATTATCTGGTTACGCACTCCCAACTGCCGA
AGGCAGATACTGCTTGATGGCTTTGCCCGCCTATGGCCACAAATGCTGGATAGACTGAATCAGGGCGAGATGCTCATCGA
GCTGAGAAGCTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8073.21 Da
• Isoelectric Point: 4.6400

>AT25588 WP_014027920.1 NC_015942:431965-432186 [Acidithiobacillus ferrivorans SS3]
MQLERITINPHQCGGRPCIRGLRVRVQDILELLAAGATTAEILEDYPYLEPEDIAASLHYAAQQADHAVLYVA

Nucleotide

• Length: 222 bp

>AT25588 NC_015942:431965-432186 [Acidithiobacillus ferrivorans SS3]
ATGCAGCTTGAGCGCATCACTATCAATCCCCATCAATGTGGCGGACGGCCTTGTATTCGAGGGCTGCGTGTTCGGGTACA
GGATATTCTCGAACTTTTGGCGGCGGGAGCGACGACTGCAGAGATTCTGGAAGATTACCCCTACCTTGAGCCCGAAGATA
TCGCCGCCAGCCTTCATTACGCTGCGCAGCAAGCGGACCACGCCGTTCTTTATGTGGCCTGA

Structures

Toxin

• AlphaFold DB: G0JT96

Antitoxin

• AlphaFold DB: G0JT95