Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 263202..263833 Replicon chromosome
Accession NZ_CP103623
Organism Escherichia coli strain 3985

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag M5T36_RS01195 Protein ID WP_001259388.1
Coordinates 263202..263477 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag M5T36_RS01200 Protein ID WP_000593555.1
Coordinates 263474..263833 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T36_RS01180 (258206) 258206..259273 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
M5T36_RS01185 (259270) 259270..262005 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
M5T36_RS01190 (262005) 262005..263129 + 1125 WP_001615305.1 ABC transporter permease -
M5T36_RS01195 (263202) 263202..263477 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
M5T36_RS01200 (263474) 263474..263833 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
M5T36_RS01205 (263953) 263953..264354 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
M5T36_RS01210 (264360) 264360..265166 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
M5T36_RS01215 (265163) 265163..265927 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
M5T36_RS01220 (265927) 265927..266760 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
M5T36_RS01225 (266757) 266757..267701 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T255871 WP_001259388.1 NZ_CP103623:263202-263477 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT255871 WP_000593555.1 NZ_CP103623:263474-263833 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References