Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5334065..5334690 Replicon chromosome
Accession NZ_CP103618
Organism Klebsiella pneumoniae strain 4383

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M5T08_RS25795 Protein ID WP_002882817.1
Coordinates 5334065..5334448 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5T08_RS25800 Protein ID WP_004150355.1
Coordinates 5334448..5334690 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T08_RS25780 (5331431) 5331431..5332333 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5T08_RS25785 (5332330) 5332330..5332965 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M5T08_RS25790 (5332962) 5332962..5333891 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5T08_RS25795 (5334065) 5334065..5334448 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M5T08_RS25800 (5334448) 5334448..5334690 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5T08_RS25805 (5334895) 5334895..5335812 + 918 WP_004181612.1 alpha/beta hydrolase -
M5T08_RS25810 (5335826) 5335826..5336767 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5T08_RS25815 (5336812) 5336812..5337249 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5T08_RS25820 (5337246) 5337246..5338106 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5T08_RS25825 (5338100) 5338100..5338699 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T255854 WP_002882817.1 NZ_CP103618:c5334448-5334065 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255854 WP_004150355.1 NZ_CP103618:c5334690-5334448 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References