Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4736454..4737264 Replicon chromosome
Accession NZ_CP103618
Organism Klebsiella pneumoniae strain 4383

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5T08_RS22975 Protein ID WP_004178461.1
Coordinates 4736454..4736987 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5T08_RS22980 Protein ID WP_002887278.1
Coordinates 4736998..4737264 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T08_RS22970 (4735285) 4735285..4736406 + 1122 WP_002887282.1 cupin domain-containing protein -
M5T08_RS22975 (4736454) 4736454..4736987 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5T08_RS22980 (4736998) 4736998..4737264 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5T08_RS22985 (4737367) 4737367..4738800 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
M5T08_RS22990 (4738790) 4738790..4739473 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M5T08_RS22995 (4739645) 4739645..4741030 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
M5T08_RS23000 (4741048) 4741048..4741392 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T255850 WP_004178461.1 NZ_CP103618:c4736987-4736454 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT255850 WP_002887278.1 NZ_CP103618:c4737264-4736998 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References