Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 592475..593051 Replicon chromosome
Accession NZ_CP103611
Organism Enterobacter cloacae strain 3143

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag M5S62_RS02845 Protein ID WP_023620650.1
Coordinates 592475..592762 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID -
Locus tag M5S62_RS02850 Protein ID WP_057072722.1
Coordinates 592749..593051 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S62_RS02820 (M5S62_02820) 587538..588233 + 696 WP_045295461.1 winged helix-turn-helix domain-containing protein -
M5S62_RS02825 (M5S62_02825) 588437..588907 + 471 WP_013095432.1 MarR family transcriptional regulator -
M5S62_RS02830 (M5S62_02830) 588904..589971 + 1068 WP_029882194.1 HlyD family secretion protein -
M5S62_RS02835 (M5S62_02835) 589961..591037 + 1077 WP_013095434.1 DUF2955 domain-containing protein -
M5S62_RS02840 (M5S62_02840) 591007..592305 - 1299 WP_013095435.1 DUF445 domain-containing protein -
M5S62_RS02845 (M5S62_02845) 592475..592762 + 288 WP_023620650.1 BrnT family toxin Toxin
M5S62_RS02850 (M5S62_02850) 592749..593051 + 303 WP_057072722.1 BrnA antitoxin family protein Antitoxin
M5S62_RS02855 (M5S62_02855) 593082..593720 - 639 WP_129327048.1 LysE family translocator -
M5S62_RS02860 (M5S62_02860) 593769..594512 - 744 WP_057071674.1 AraC family transcriptional regulator -
M5S62_RS02865 (M5S62_02865) 594650..596020 + 1371 WP_038418628.1 NAD-dependent succinate-semialdehyde dehydrogenase -
M5S62_RS02870 (M5S62_02870) 596062..596382 - 321 WP_013095439.1 hypothetical protein -
M5S62_RS02875 (M5S62_02875) 596382..596927 - 546 WP_013095440.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T255814 WP_023620650.1 NZ_CP103611:592475-592762 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11418.04 Da        Isoelectric Point: 10.3694

>AT255814 WP_057072722.1 NZ_CP103611:592749-593051 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKTEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure

References