Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 83465..84066 Replicon plasmid pMB7206_1
Accession NZ_CP103610
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag M5S83_RS24020 Protein ID WP_001216034.1
Coordinates 83465..83845 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag M5S83_RS24025 Protein ID WP_001190712.1
Coordinates 83845..84066 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS23990 (M5S83_23990) 78565..78684 - 120 WP_071527722.1 ash family protein -
M5S83_RS23995 (M5S83_23995) 78906..80390 - 1485 WP_000124159.1 hypothetical protein -
M5S83_RS24000 (M5S83_24000) 80390..81583 - 1194 WP_166465177.1 terminase -
M5S83_RS24005 (M5S83_24005) 81669..82121 - 453 WP_001326849.1 late promoter-activating protein -
M5S83_RS24010 (M5S83_24010) 82210..83253 - 1044 WP_001561086.1 DUF968 domain-containing protein -
M5S83_RS24015 (M5S83_24015) 83281..83460 - 180 WP_000113018.1 hypothetical protein -
M5S83_RS24020 (M5S83_24020) 83465..83845 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
M5S83_RS24025 (M5S83_24025) 83845..84066 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M5S83_RS24030 (M5S83_24030) 84249..85805 + 1557 WP_166465176.1 type I restriction-modification system subunit M -
M5S83_RS24035 (M5S83_24035) 85802..87004 + 1203 WP_166465175.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..98694 98694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T255810 WP_001216034.1 NZ_CP103610:c83845-83465 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT255810 WP_001190712.1 NZ_CP103610:c84066-83845 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References