Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4051223..4051877 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag M5S83_RS19490 Protein ID WP_000244772.1
Coordinates 4051223..4051630 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M5S83_RS19495 Protein ID WP_000354046.1
Coordinates 4051611..4051877 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS19470 (4047180) 4047180..4048913 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
M5S83_RS19475 (4048919) 4048919..4049629 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M5S83_RS19480 (4049654) 4049654..4050550 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
M5S83_RS19485 (4050662) 4050662..4051183 + 522 WP_001055874.1 flavodoxin FldB -
M5S83_RS19490 (4051223) 4051223..4051630 - 408 WP_000244772.1 protein YgfX Toxin
M5S83_RS19495 (4051611) 4051611..4051877 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M5S83_RS19500 (4052120) 4052120..4053100 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
M5S83_RS19505 (4053177) 4053177..4053836 - 660 WP_000250274.1 hemolysin III family protein -
M5S83_RS19510 (4054000) 4054000..4054311 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
M5S83_RS19515 (4054356) 4054356..4055789 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
M5S83_RS19520 (4055846) 4055846..4056589 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T255807 WP_000244772.1 NZ_CP103609:c4051630-4051223 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT255807 WP_000354046.1 NZ_CP103609:c4051877-4051611 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References