Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3873956..3874539 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag M5S83_RS18720 Protein ID WP_000254745.1
Coordinates 3873956..3874291 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag M5S83_RS18725 Protein ID WP_000581937.1
Coordinates 3874291..3874539 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS18705 (3869843) 3869843..3871141 - 1299 WP_000036723.1 phosphopyruvate hydratase -
M5S83_RS18710 (3871229) 3871229..3872866 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
M5S83_RS18715 (3873094) 3873094..3873885 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
M5S83_RS18720 (3873956) 3873956..3874291 - 336 WP_000254745.1 endoribonuclease MazF Toxin
M5S83_RS18725 (3874291) 3874291..3874539 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
M5S83_RS18730 (3874617) 3874617..3876851 - 2235 WP_000226815.1 GTP pyrophosphokinase -
M5S83_RS18735 (3876899) 3876899..3878206 - 1308 WP_259387808.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T255806 WP_000254745.1 NZ_CP103609:c3874291-3873956 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT255806 WP_000581937.1 NZ_CP103609:c3874539-3874291 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References