Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3542461..3543086 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag M5S83_RS17050 Protein ID WP_000911329.1
Coordinates 3542461..3542859 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag M5S83_RS17055 Protein ID WP_000450524.1
Coordinates 3542859..3543086 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS17030 (3538339) 3538339..3538539 + 201 WP_000383836.1 YpfN family protein -
M5S83_RS17035 (3538649) 3538649..3539347 - 699 WP_000679823.1 esterase -
M5S83_RS17040 (3539421) 3539421..3541436 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
M5S83_RS17045 (3541451) 3541451..3542314 - 864 WP_001267498.1 neutral zinc metallopeptidase -
M5S83_RS17050 (3542461) 3542461..3542859 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S83_RS17055 (3542859) 3542859..3543086 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
M5S83_RS17060 (3543240) 3543240..3543953 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
M5S83_RS17065 (3544166) 3544166..3545200 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
M5S83_RS17070 (3545217) 3545217..3546095 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
M5S83_RS17075 (3546241) 3546241..3546813 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
M5S83_RS17080 (3546813) 3546813..3547283 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T255805 WP_000911329.1 NZ_CP103609:c3542859-3542461 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT255805 WP_000450524.1 NZ_CP103609:c3543086-3542859 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References