Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 810227..810803 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag M5S83_RS03955 Protein ID WP_001350372.1
Coordinates 810227..810514 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag M5S83_RS03960 Protein ID WP_001402821.1
Coordinates 810501..810803 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS03940 (805809) 805809..807191 - 1383 WP_023155808.1 restriction endonuclease subunit S -
M5S83_RS03945 (807181) 807181..808800 - 1620 WP_001029742.1 class I SAM-dependent DNA methyltransferase -
M5S83_RS03950 (808864) 808864..810030 - 1167 WP_001672354.1 restriction endonuclease -
M5S83_RS03955 (810227) 810227..810514 + 288 WP_001350372.1 BrnT family toxin Toxin
M5S83_RS03960 (810501) 810501..810803 + 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
M5S83_RS03965 (810837) 810837..811793 - 957 WP_001295525.1 GTPase -
M5S83_RS03970 (811804) 811804..812007 - 204 WP_000467859.1 YbdD/YjiX family protein -
M5S83_RS03975 (812057) 812057..814207 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
M5S83_RS03980 (814600) 814600..815112 - 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T255794 WP_001350372.1 NZ_CP103609:810227-810514 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT255794 WP_001402821.1 NZ_CP103609:810501-810803 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References