Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 576049..576546 Replicon chromosome
Accession NZ_CP103609
Organism Escherichia coli strain 4314

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5S83_RS02770 Protein ID WP_001173343.1
Coordinates 576373..576546 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M5S83_RS02765 Protein ID WP_000398619.1
Coordinates 576049..576345 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S83_RS02745 (572780) 572780..573499 - 720 WP_000611288.1 two-component system response regulator DcuR -
M5S83_RS02750 (573496) 573496..575127 - 1632 WP_001216460.1 sensor histidine kinase -
M5S83_RS02755 (575308) 575308..575538 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
M5S83_RS02760 (575550) 575550..575822 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5S83_RS02765 (576049) 576049..576345 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5S83_RS02770 (576373) 576373..576546 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5S83_RS02775 (576665) 576665..578182 - 1518 WP_023155344.1 lysine--tRNA ligase -
M5S83_RS02780 (578419) 578419..579876 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255792 WP_001173343.1 NZ_CP103609:576373-576546 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT255792 WP_000398619.1 NZ_CP103609:576049-576345 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References