Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5150989..5151614 Replicon chromosome
Accession NZ_CP103605
Organism Klebsiella pneumoniae strain 4386

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag M5S85_RS24885 Protein ID WP_019705794.1
Coordinates 5150989..5151372 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5S85_RS24890 Protein ID WP_004150355.1
Coordinates 5151372..5151614 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S85_RS24870 (5148355) 5148355..5149257 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5S85_RS24875 (5149254) 5149254..5149889 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
M5S85_RS24880 (5149886) 5149886..5150815 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5S85_RS24885 (5150989) 5150989..5151372 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S85_RS24890 (5151372) 5151372..5151614 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5S85_RS24895 (5151819) 5151819..5152736 + 918 WP_032430300.1 alpha/beta hydrolase -
M5S85_RS24900 (5152751) 5152751..5153692 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5S85_RS24905 (5153737) 5153737..5154174 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5S85_RS24910 (5154171) 5154171..5155031 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5S85_RS24915 (5155025) 5155025..5155624 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T255788 WP_019705794.1 NZ_CP103605:c5151372-5150989 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255788 WP_004150355.1 NZ_CP103605:c5151614-5151372 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References