Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3987548..3988124 Replicon chromosome
Accession NZ_CP103596
Organism Escherichia coli strain 4848

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5T19_RS19365 Protein ID WP_001297643.1
Coordinates 3987837..3988124 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5T19_RS19360 Protein ID WP_000063156.1
Coordinates 3987548..3987850 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T19_RS19345 (3984144) 3984144..3986294 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5T19_RS19350 (3986344) 3986344..3986547 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5T19_RS19355 (3986558) 3986558..3987514 + 957 WP_001297640.1 GTPase -
M5T19_RS19360 (3987548) 3987548..3987850 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5T19_RS19365 (3987837) 3987837..3988124 - 288 WP_001297643.1 BrnT family toxin Toxin
M5T19_RS19370 (3988323) 3988323..3989020 - 698 Protein_3784 IS1 family transposase -
M5T19_RS19375 (3989077) 3989077..3989172 + 96 Protein_3785 dienelactone hydrolase family protein -
M5T19_RS19380 (3989318) 3989318..3990550 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M5T19_RS19385 (3990591) 3990591..3991871 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255768 WP_001297643.1 NZ_CP103596:c3988124-3987837 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT255768 WP_000063156.1 NZ_CP103596:c3987850-3987548 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References