Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 118659..119184 Replicon plasmid pMB7542_1
Accession NZ_CP103593
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag M5S53_RS25670 Protein ID WP_001159868.1
Coordinates 118659..118964 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag M5S53_RS25675 Protein ID WP_000813634.1
Coordinates 118966..119184 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS25650 (114500) 114500..116008 + 1509 WP_001189113.1 group II intron reverse transcriptase/maturase -
M5S53_RS25655 (116092) 116092..116217 + 126 Protein_142 IS3 family transposase -
M5S53_RS25660 (116270) 116270..116974 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
M5S53_RS25665 (117852) 117852..118658 - 807 WP_000016982.1 site-specific integrase -
M5S53_RS25670 (118659) 118659..118964 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
M5S53_RS25675 (118966) 118966..119184 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
M5S53_RS25680 (119892) 119892..120887 + 996 WP_000246636.1 hypothetical protein -
M5S53_RS25685 (120930) 120930..121823 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..138020 138020
- inside IScluster/Tn - - 112757..122463 9706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T255750 WP_001159868.1 NZ_CP103593:c118964-118659 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT255750 WP_000813634.1 NZ_CP103593:c119184-118966 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References