Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4555859..4556356 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5S53_RS22510 Protein ID WP_001173343.1
Coordinates 4555859..4556032 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag M5S53_RS22515 Protein ID WP_001312331.1
Coordinates 4556060..4556356 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS22500 (4552529) 4552529..4553986 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
M5S53_RS22505 (4554223) 4554223..4555740 + 1518 WP_000003806.1 lysine--tRNA ligase -
M5S53_RS22510 (4555859) 4555859..4556032 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5S53_RS22515 (4556060) 4556060..4556356 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5S53_RS22520 (4556584) 4556584..4556856 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5S53_RS22525 (4557070) 4557070..4560999 - 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4547488..4571947 24459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255741 WP_001173343.1 NZ_CP103592:c4556032-4555859 [Escherichia coli O25b:H4-ST131]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT255741 WP_001312331.1 NZ_CP103592:c4556356-4556060 [Escherichia coli O25b:H4-ST131]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References