Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4452759..4453354 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag M5S53_RS21980 Protein ID WP_000239579.1
Coordinates 4452759..4453109 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag M5S53_RS21985 Protein ID WP_001223208.1
Coordinates 4453103..4453354 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS21960 (4448205) 4448205..4449227 - 1023 WP_001296689.1 ABC transporter permease -
M5S53_RS21965 (4449241) 4449241..4450743 - 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
M5S53_RS21970 (4450883) 4450883..4451839 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
M5S53_RS21975 (4452149) 4452149..4452679 + 531 WP_000055075.1 inorganic diphosphatase -
M5S53_RS21980 (4452759) 4452759..4453109 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
M5S53_RS21985 (4453103) 4453103..4453354 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
M5S53_RS21990 (4453566) 4453566..4453907 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
M5S53_RS21995 (4453910) 4453910..4457689 - 3780 WP_000060945.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T255740 WP_000239579.1 NZ_CP103592:c4453109-4452759 [Escherichia coli O25b:H4-ST131]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT255740 WP_001223208.1 NZ_CP103592:c4453354-4453103 [Escherichia coli O25b:H4-ST131]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References