Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4270425..4271001 Replicon chromosome
Accession NZ_CP103592
Organism Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag M5S53_RS21060 Protein ID WP_001295743.1
Coordinates 4270714..4271001 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag M5S53_RS21055 Protein ID WP_000063148.1
Coordinates 4270425..4270727 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S53_RS21040 (4267021) 4267021..4269171 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S53_RS21045 (4269221) 4269221..4269424 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S53_RS21050 (4269435) 4269435..4270391 + 957 WP_001295745.1 GTPase -
M5S53_RS21055 (4270425) 4270425..4270727 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
M5S53_RS21060 (4270714) 4270714..4271001 - 288 WP_001295743.1 BrnT family toxin Toxin
M5S53_RS21065 (4271198) 4271198..4272364 + 1167 WP_000800831.1 restriction endonuclease -
M5S53_RS21070 (4272428) 4272428..4274047 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
M5S53_RS21075 (4274037) 4274037..4275341 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T255738 WP_001295743.1 NZ_CP103592:c4271001-4270714 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT255738 WP_000063148.1 NZ_CP103592:c4270727-4270425 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References