Detailed information of TA system TA255736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3892835..3894524
• Replicon: chromosome
• Accession: NZ_CP103592
• Organism: Escherichia coli O25b:H4-ST131 strain 4224

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: M5S53_RS19240
• Protein ID: WP_000365807.1
• Coordinates: 3892835..3893755 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5S53_RS19245
• Protein ID: WP_001295795.1
• Coordinates: 3893742..3894524 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5S53_RS19200 (3888308)	3888308..3889018	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
M5S53_RS19205 (3889325)	3889325..3889654	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
M5S53_RS19210 (3889666)	3889666..3889832	-	167	Protein_3772	tail fiber assembly protein	-
M5S53_RS19215 (3889903)	3889903..3890517	-	615	WP_001019923.1	YagU family protein	-
M5S53_RS19220 (3890732)	3890732..3890917	-	186	WP_000146240.1	hypothetical protein	-
M5S53_RS19225 (3890966)	3890966..3891124	+	159	Protein_3775	integrase	-
M5S53_RS19230 (3891351)	3891351..3891686	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
M5S53_RS19235 (3891894)	3891894..3892670	+	777	WP_000171155.1	site-specific integrase	-
M5S53_RS19240 (3892835)	3892835..3893755	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5S53_RS19245 (3893742)	3893742..3894524	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5S53_RS19250 (3894808)	3894808..3897906	+	3099	WP_000673070.1	type I restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3852619..3901587	48968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T255736 WP_000365807.1 NZ_CP103592:c3893755-3892835 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT255736 WP_001295795.1 NZ_CP103592:c3894524-3893742 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0