Detailed information of TA system TA025573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 968891..969450
• Replicon: chromosome
• Accession: NC_015857
• Organism: Brucella pinnipedialis B2/94

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BPI_RS04765
• Protein ID: WP_004688321.1
• Coordinates: 969205..969450 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D0RHG9
• Locus tag: BPI_RS04760
• Protein ID: WP_006161014.1
• Coordinates: 968891..969205 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPI_RS04715	964582..964785	-	204	WP_002964087.1	hypothetical protein	-
BPI_RS04720	964788..965003	-	216	WP_002971459.1	hypothetical protein	-
BPI_RS04725	965000..965203	-	204	WP_002964088.1	hypothetical protein	-
BPI_RS04730	965253..965972	+	720	WP_006072696.1	hypothetical protein	-
BPI_RS04735	965969..966199	+	231	WP_002964091.1	hypothetical protein	-
BPI_RS04740	966196..966471	+	276	WP_002964092.1	hypothetical protein	-
BPI_RS04745	966494..967081	+	588	WP_004683738.1	hypothetical protein	-
BPI_RS04750	967316..968025	+	710	Protein_923	porin family protein	-
BPI_RS04755	968373..968543	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BPI_RS16865	968542..968775	+	234	WP_002969999.1	hypothetical protein	-
BPI_RS04760	968891..969205	-	315	WP_006161014.1	BrnA antitoxin family protein	Antitoxin
BPI_RS04765	969205..969450	-	246	WP_004688321.1	BrnT family toxin	Toxin
BPI_RS04770	969583..970221	+	639	Protein_928	tRNA-guanine transglycosylase	-
BPI_RS18085	970291..971048	+	758	WP_134790775.1	IS5 family transposase	-
BPI_RS04785	971049..971822	+	774	WP_002967634.1	hypothetical protein	-
BPI_RS04790	971957..973603	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	963571..976919	13348

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T25573 WP_004688321.1 NC_015857:c969450-969205 [Brucella pinnipedialis B2/94]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T25573 NC_015857:c969450-969205 [Brucella pinnipedialis B2/94]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11668.45 Da
• Isoelectric Point: 10.0945

>AT25573 WP_006161014.1 NC_015857:c969205-968891 [Brucella pinnipedialis B2/94]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGWPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT25573 NC_015857:c969205-968891 [Brucella pinnipedialis B2/94]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCTGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Antitoxin

• AlphaFold DB: D0RHG9