Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4647043..4647540 Replicon chromosome
Accession NZ_CP103589
Organism Escherichia coli strain 4750

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5T00_RS22635 Protein ID WP_001173343.1
Coordinates 4647043..4647216 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag M5T00_RS22640 Protein ID WP_001312331.1
Coordinates 4647244..4647540 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T00_RS22625 (4643713) 4643713..4645170 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
M5T00_RS22630 (4645407) 4645407..4646924 + 1518 WP_000003806.1 lysine--tRNA ligase -
M5T00_RS22635 (4647043) 4647043..4647216 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5T00_RS22640 (4647244) 4647244..4647540 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5T00_RS22645 (4647769) 4647769..4648041 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5T00_RS22650 (4648053) 4648053..4648283 - 231 WP_000373293.1 4Fe-4S mono-cluster protein YjdI -
M5T00_RS22655 (4648464) 4648464..4650095 + 1632 WP_001216482.1 sensor histidine kinase -
M5T00_RS22660 (4650092) 4650092..4650811 + 720 WP_001540474.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4591243..4647540 56297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255715 WP_001173343.1 NZ_CP103589:c4647216-4647043 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT255715 WP_001312331.1 NZ_CP103589:c4647540-4647244 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References