Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1111607..1112190 Replicon chromosome
Accession NZ_CP103589
Organism Escherichia coli strain 4750

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag M5T00_RS05295 Protein ID WP_000254749.1
Coordinates 1111855..1112190 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag M5T00_RS05290 Protein ID WP_000581937.1
Coordinates 1111607..1111855 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T00_RS05280 (1107946) 1107946..1109247 + 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
M5T00_RS05285 (1109295) 1109295..1111529 + 2235 WP_000226797.1 GTP diphosphokinase -
M5T00_RS05290 (1111607) 1111607..1111855 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
M5T00_RS05295 (1111855) 1111855..1112190 + 336 WP_000254749.1 endoribonuclease MazF Toxin
M5T00_RS05300 (1112262) 1112262..1113053 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
M5T00_RS05305 (1113281) 1113281..1114918 + 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
M5T00_RS05310 (1115006) 1115006..1116304 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T255697 WP_000254749.1 NZ_CP103589:1111855-1112190 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT255697 WP_000581937.1 NZ_CP103589:1111607-1111855 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References