Detailed information of TA system TA025565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-parD/RelE-RHH
• Location: 36985..37556
• Replicon: plasmid megaplasmid
• Accession: NC_015851
• Organism: Acidithiobacillus caldus SM-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F9ZUQ2
• Locus tag: ATC_RS14620
• Protein ID: WP_014003668.1
• Coordinates: 37272..37556 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F9ZUQ1
• Locus tag: ATC_RS14615
• Protein ID: WP_014003667.1
• Coordinates: 36985..37275 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATC_RS17160	32486..32623	-	138	Protein_33	IS3 family transposase	-
ATC_RS17165	32592..32714	+	123	WP_187288691.1	transposase	-
ATC_RS14580	32860..33324	-	465	WP_014003661.1	SOS-response repressor and protease LexA	-
ATC_RS14585	33348..33653	-	306	WP_148262249.1	hypothetical protein	-
ATC_RS16085	33622..33912	-	291	WP_148262250.1	hypothetical protein	-
ATC_RS14595	34180..35112	+	933	WP_014003663.1	tyrosine-type recombinase/integrase	-
ATC_RS14600	35705..35980	+	276	WP_014003664.1	HU family DNA-binding protein	-
ATC_RS14605	35992..36354	+	363	WP_014003665.1	hypothetical protein	-
ATC_RS14610	36445..36756	+	312	WP_014003666.1	integration host factor subunit alpha	-
ATC_RS14615	36985..37275	+	291	WP_014003667.1	hypothetical protein	Antitoxin
ATC_RS14620	37272..37556	+	285	WP_014003668.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
ATC_RS16090	37833..38429	-	597	WP_103415156.1	uracil-DNA glycosylase	-
ATC_RS14630	38426..38659	-	234	WP_014003670.1	hypothetical protein	-
ATC_RS14635	38674..39371	-	698	Protein_46	prepilin peptidase	-
ATC_RS14640	39447..39779	-	333	WP_014003673.1	hypothetical protein	-
ATC_RS14645	39831..40877	-	1047	WP_014003674.1	IS630 family transposase	-
ATC_RS14650	40928..42262	+	1335	WP_014003675.1	type II toxin-antitoxin system HipA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..251782	251782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10643.24 Da
• Isoelectric Point: 6.8795

>T25565 WP_014003668.1 NC_015851:37272-37556 [Acidithiobacillus caldus SM-1]
VKLFWTPEAIQDRDDIYAHIEADNPAAALALDELFSEKAARLVDHPGQGRSGRVVGTRELVVHRNYILVYDLAEGMVRVL
RVLHAARQWPPVKM

Nucleotide

• Length: 285 bp

>T25565 NC_015851:37272-37556 [Acidithiobacillus caldus SM-1]
GTGAAACTGTTCTGGACACCAGAGGCGATACAAGACCGCGACGACATCTATGCCCATATCGAGGCCGACAATCCGGCCGC
CGCACTGGCTCTCGATGAGCTGTTTTCAGAGAAGGCTGCTCGCCTGGTAGATCACCCAGGTCAGGGCCGGTCTGGGCGCG
TTGTCGGCACCCGCGAGCTGGTAGTGCATAGGAATTACATTCTGGTCTATGACTTGGCAGAGGGCATGGTTCGCGTGTTG
CGTGTCCTGCACGCGGCTCGACAGTGGCCACCAGTGAAGATGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10989.39 Da
• Isoelectric Point: 10.2812

>AT25565 WP_014003667.1 NC_015851:36985-37275 [Acidithiobacillus caldus SM-1]
MSEATFTFRVDEALKIEFSKAAKARDRSGAQLLRDFMREFVQQQQEATAHDAWFRRQVQIGMDSANAGNLIPAAEVEARF
AAKRVATRRRLEASEW

Nucleotide

• Length: 291 bp

>AT25565 NC_015851:36985-37275 [Acidithiobacillus caldus SM-1]
ATGAGCGAAGCTACCTTTACTTTTCGGGTCGATGAAGCCTTGAAAATCGAGTTCTCCAAGGCCGCTAAGGCGCGCGACCG
CAGCGGTGCGCAACTCCTGCGCGACTTCATGCGTGAGTTCGTGCAGCAGCAACAGGAAGCGACCGCGCATGACGCATGGT
TCCGTCGCCAGGTGCAAATTGGCATGGATTCGGCTAATGCAGGTAACCTGATTCCAGCGGCCGAGGTGGAAGCCAGGTTT
GCGGCCAAGCGGGTGGCCACGCGTCGCCGACTTGAAGCCTCCGAATGGTGA

Structures

Toxin

• AlphaFold DB: F9ZUQ2

Antitoxin

• AlphaFold DB: F9ZUQ1