Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2432542..2434165 Replicon chromosome
Accession NZ_CP103577
Organism Enterobacter hormaechei strain 4285

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag M5S64_RS11600 Protein ID WP_000088044.1
Coordinates 2432542..2433867 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag M5S64_RS11605 Protein ID WP_012477377.1
Coordinates 2433872..2434165 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S64_RS11570 (M5S64_11570) 2428135..2428896 + 762 WP_002210513.1 DeoR/GlpR family DNA-binding transcription regulator -
M5S64_RS11575 (M5S64_11575) 2428917..2429777 - 861 WP_002904004.1 extended-spectrum class A beta-lactamase SHV-12 -
M5S64_RS11580 (M5S64_11580) 2429914..2430618 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
M5S64_RS11585 (M5S64_11585) 2430936..2431709 - 774 WP_001139513.1 HNH endonuclease signature motif containing protein -
M5S64_RS11590 (M5S64_11590) 2431764..2432174 - 411 WP_000985909.1 hypothetical protein -
M5S64_RS11595 (M5S64_11595) 2432275..2432481 - 207 WP_000134171.1 hypothetical protein -
M5S64_RS11600 (M5S64_11600) 2432542..2433867 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
M5S64_RS11605 (M5S64_11605) 2433872..2434165 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
M5S64_RS11610 (M5S64_11610) 2434566..2435954 - 1389 WP_000083579.1 hypothetical protein -
M5S64_RS11615 (M5S64_11615) 2435959..2436456 - 498 WP_000340139.1 membrane protein -
M5S64_RS11620 (M5S64_11620) 2436659..2437315 - 657 WP_000462754.1 trypsin-like serine protease -
M5S64_RS11625 (M5S64_11625) 2437974..2438366 - 393 WP_000341066.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage qnrA1 / sul1 / blaSHV-12 - 2416469..2441468 24999
- flank IS/Tn - - 2439108..2439812 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T255640 WP_000088044.1 NZ_CP103577:c2433867-2432542 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT255640 WP_012477377.1 NZ_CP103577:c2434165-2433872 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References