Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3969775..3970351 Replicon chromosome
Accession NZ_CP103570
Organism Escherichia coli strain 3045

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M5S79_RS19070 Protein ID WP_001297643.1
Coordinates 3970064..3970351 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M5S79_RS19065 Protein ID WP_000063156.1
Coordinates 3969775..3970077 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S79_RS19050 (3966371) 3966371..3968521 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M5S79_RS19055 (3968571) 3968571..3968774 + 204 WP_000467859.1 YbdD/YjiX family protein -
M5S79_RS19060 (3968785) 3968785..3969741 + 957 WP_001297640.1 GTPase -
M5S79_RS19065 (3969775) 3969775..3970077 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M5S79_RS19070 (3970064) 3970064..3970351 - 288 WP_001297643.1 BrnT family toxin Toxin
M5S79_RS19075 (3970550) 3970550..3971247 - 698 Protein_3727 IS1 family transposase -
M5S79_RS19080 (3971304) 3971304..3971399 + 96 Protein_3728 dienelactone hydrolase family protein -
M5S79_RS19085 (3971545) 3971545..3972777 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M5S79_RS19090 (3972818) 3972818..3974098 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T255621 WP_001297643.1 NZ_CP103570:c3970351-3970064 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT255621 WP_000063156.1 NZ_CP103570:c3970077-3969775 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References