Detailed information of TA system TA025562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1590807..1591402
• Replicon: chromosome
• Accession: NC_015850
• Organism: Acidithiobacillus caldus SM-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F9ZNF3
• Locus tag: ATC_RS07725
• Protein ID: WP_014002921.1
• Coordinates: 1590807..1591103 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: ATC_RS07730
• Protein ID: WP_004872065.1
• Coordinates: 1591109..1591402 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATC_RS07705	1586262..1587179	+	918	WP_004872060.1	magnesium transporter	-
ATC_RS07710	1587201..1588394	-	1194	WP_004872061.1	divalent metal cation transporter	-
ATC_RS07715	1588654..1589361	-	708	WP_004872063.1	MgtC/SapB family protein	-
ATC_RS16430	1589425..1589634	-	210	WP_103415044.1	hypothetical protein	-
ATC_RS07725	1590807..1591103	+	297	WP_014002921.1	addiction module antitoxin RelB	Toxin
ATC_RS07730	1591109..1591402	+	294	WP_004872065.1	putative addiction module antidote protein	Antitoxin
ATC_RS07735	1591638..1592597	-	960	WP_103415125.1	GTP-binding protein	-
ATC_RS07740	1592921..1594546	-	1626	WP_014002923.1	GGDEF domain-containing protein	-
ATC_RS07745	1594594..1594980	-	387	WP_049784663.1	GGDEF domain-containing protein	-
ATC_RS07750	1595012..1596226	-	1215	WP_012387019.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1595012..1596226	1214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11235.09 Da
• Isoelectric Point: 9.9555

>T25562 WP_014002921.1 NC_015850:1590807-1591103 [Acidithiobacillus caldus SM-1]
MKEIIKSDIFDRWLIALRDRRARARIEVRIRRLSLGNPGDVKPVGEGVLEIRIDYDPGYHVYFMSRGPLVIVLLCAGDKS
SQDRDIALAKNIAAQWKE

Nucleotide

• Length: 297 bp

>T25562 NC_015850:1590807-1591103 [Acidithiobacillus caldus SM-1]
ATGAAGGAAATCATCAAATCCGATATTTTCGATCGCTGGCTTATCGCACTTCGTGACCGACGAGCCCGAGCAAGGATCGA
GGTGCGAATTCGGCGATTGAGCCTTGGTAATCCCGGAGATGTAAAACCAGTAGGGGAAGGCGTATTGGAAATACGGATTG
ATTACGATCCAGGTTACCACGTCTATTTTATGAGTCGTGGCCCATTGGTCATAGTACTGCTTTGTGCTGGCGACAAAAGC
AGTCAGGACCGGGATATTGCCTTGGCCAAGAACATTGCAGCACAGTGGAAGGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10471.97 Da
• Isoelectric Point: 5.0634

>AT25562 WP_004872065.1 NC_015850:1591109-1591402 [Acidithiobacillus caldus SM-1]
MDIQFSRYDTVDYLQTEEDMVAYLDAVMEDGDPALIAAALGDIARARNLSRLAREVGMSRQGLDKALSGKGNPSLATVLK
VAKALGLRLAFQTARQG

Nucleotide

• Length: 294 bp

>AT25562 NC_015850:1591109-1591402 [Acidithiobacillus caldus SM-1]
ATGGACATCCAATTCAGTCGCTATGACACGGTAGACTACCTTCAGACCGAGGAAGACATGGTGGCTTATCTCGATGCGGT
GATGGAAGACGGCGACCCTGCATTGATTGCCGCAGCGCTGGGGGACATAGCCCGCGCCCGCAATCTCAGCCGCCTCGCCC
GAGAAGTCGGAATGAGCCGCCAGGGGCTGGACAAGGCCCTTTCGGGCAAAGGCAACCCCAGCTTGGCTACGGTCCTCAAG
GTCGCCAAGGCGCTCGGGCTAAGACTGGCCTTTCAAACAGCCAGACAAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A1E7YSJ9