Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5245503..5246128 Replicon chromosome
Accession NZ_CP103567
Organism Klebsiella pneumoniae strain 4680

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag M5S58_RS25390 Protein ID WP_019705794.1
Coordinates 5245503..5245886 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M5S58_RS25395 Protein ID WP_004150355.1
Coordinates 5245886..5246128 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S58_RS25375 (5242869) 5242869..5243771 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M5S58_RS25380 (5243768) 5243768..5244403 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
M5S58_RS25385 (5244400) 5244400..5245329 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M5S58_RS25390 (5245503) 5245503..5245886 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
M5S58_RS25395 (5245886) 5245886..5246128 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M5S58_RS25400 (5246333) 5246333..5247250 + 918 WP_032430300.1 alpha/beta hydrolase -
M5S58_RS25405 (5247265) 5247265..5248206 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M5S58_RS25410 (5248251) 5248251..5248688 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M5S58_RS25415 (5248685) 5248685..5249545 - 861 WP_004146232.1 virulence factor BrkB family protein -
M5S58_RS25420 (5249539) 5249539..5250138 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T255605 WP_019705794.1 NZ_CP103567:c5245886-5245503 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT255605 WP_004150355.1 NZ_CP103567:c5246128-5245886 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References