Detailed information of TA system TA025559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4060663..4061329
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MCAN_RS18790
• Protein ID: WP_015303952.1
• Coordinates: 4060898..4061329 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS18785
• Protein ID: WP_041180166.1
• Coordinates: 4060663..4060911 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS18760	4056474..4057433	+	960	WP_014001808.1	acyl-CoA/acyl-ACP dehydrogenase	-
MCAN_RS18765	4057430..4058386	+	957	WP_003419352.1	acyl-CoA/acyl-ACP dehydrogenase	-
MCAN_RS18770	4058383..4059549	+	1167	WP_014001809.1	pyridoxal phosphate-dependent aminotransferase	-
MCAN_RS18775	4059514..4060365	-	852	WP_014001810.1	arylamine N-acetyltransferase	-
MCAN_RS18780	4060350..4060616	-	267	WP_003419367.1	hypothetical protein	-
MCAN_RS18785	4060663..4060911	+	249	WP_041180166.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MCAN_RS18790	4060898..4061329	+	432	WP_015303952.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS18795	4061423..4062685	-	1263	WP_014001811.1	group II intron reverse transcriptase/maturase	-
MCAN_RS18800	4063334..4063900	-	567	WP_014001812.1	flavin-dependent monooxygenase reductase subunit HsaB	-
MCAN_RS18805	4063903..4064805	-	903	WP_014001813.1	iron-dependent extradiol dioxygenase HsaC	-
MCAN_RS18810	4064802..4065677	-	876	WP_014001814.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15746.08 Da
• Isoelectric Point: 8.1635

>T25559 WP_015303952.1 NC_015848:4060898-4061329 [Mycobacterium canettii CIPT 140010059]
MLLLDVSVCIFAIREDSCPNHATYRTWLTRLLTGDEPVGISELVLSGVIRLLTNHRVFRQPSTTQQALDSCHALRSAPAA
VALRPGPRHWGIFESLCADVGARANAVPDAYHAALAIENGATWITTDRSFARFPGLRWRLPLS

Nucleotide

• Length: 432 bp

>T25559 NC_015848:4060898-4061329 [Mycobacterium canettii CIPT 140010059]
ATGCTGCTCCTTGACGTAAGCGTCTGCATATTCGCCATCCGCGAGGACAGCTGCCCCAACCACGCGACATACCGGACGTG
GCTTACCAGACTGCTTACCGGCGACGAACCCGTGGGGATAAGTGAACTCGTGCTATCCGGTGTTATTCGGCTGCTCACCA
ACCATCGGGTATTTCGGCAGCCGAGCACCACGCAGCAGGCGTTGGACTCATGCCATGCGCTGCGGTCCGCACCAGCCGCC
GTCGCGTTACGGCCAGGGCCGCGTCATTGGGGGATCTTCGAATCCCTGTGCGCCGACGTAGGCGCCCGGGCTAATGCGGT
GCCGGACGCCTATCACGCCGCACTGGCAATCGAGAACGGCGCGACGTGGATCACCACCGACCGGTCATTTGCCCGGTTTC
CGGGCCTACGCTGGCGACTCCCCCTGAGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8650.70 Da
• Isoelectric Point: 5.0484

>AT25559 WP_041180166.1 NC_015848:4060663-4060911 [Mycobacterium canettii CIPT 140010059]
MRTTVNIDDHLLAEAKILAARTSRSLGAVLEDALRAMLRRSEKGSGNSEFHLPTHGTGGLQAGVDLDDKEALAELIGDNA
AP

Nucleotide

• Length: 249 bp

>AT25559 NC_015848:4060663-4060911 [Mycobacterium canettii CIPT 140010059]
ATGCGCACCACTGTCAACATCGACGATCACCTGCTCGCCGAGGCGAAGATTCTCGCCGCGAGAACATCGCGATCGCTGGG
AGCAGTACTCGAGGATGCGCTGCGGGCCATGCTGCGGCGGTCCGAGAAGGGATCCGGCAACAGCGAGTTTCACCTGCCGA
CCCACGGCACCGGAGGCTTGCAGGCCGGTGTCGACTTGGACGACAAGGAAGCCCTCGCCGAACTGATCGGCGACAATGCT
GCTCCTTGA