Detailed information of TA system TA025558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3893530..3894215
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: MCAN_RS18045
• Protein ID: WP_003417998.1
• Coordinates: 3893805..3894215 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCAN_RS18040
• Protein ID: WP_003912220.1
• Coordinates: 3893530..3893808 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS18020	3889519..3891120	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
MCAN_RS18025	3891137..3891841	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
MCAN_RS18030	3891959..3892525	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
MCAN_RS18035	3892587..3893474	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
MCAN_RS18040	3893530..3893808	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MCAN_RS18045	3893805..3894215	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS18050	3894248..3895984	-	1737	WP_003418002.1	cholesterol oxidase	-
MCAN_RS18055	3896040..3897167	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
MCAN_RS18060	3897187..3898776	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T25558 WP_003417998.1 NC_015848:3893805-3894215 [Mycobacterium canettii CIPT 140010059]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T25558 NC_015848:3893805-3894215 [Mycobacterium canettii CIPT 140010059]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT25558 WP_003912220.1 NC_015848:3893530-3893808 [Mycobacterium canettii CIPT 140010059]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT25558 NC_015848:3893530-3893808 [Mycobacterium canettii CIPT 140010059]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTTACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9