Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4685749..4686246 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M5R24_RS23200 Protein ID WP_001173343.1
Coordinates 4685749..4685922 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag M5R24_RS23205 Protein ID WP_001312331.1
Coordinates 4685950..4686246 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS23190 (4682419) 4682419..4683876 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
M5R24_RS23195 (4684113) 4684113..4685630 + 1518 WP_000003806.1 lysine--tRNA ligase -
M5R24_RS23200 (4685749) 4685749..4685922 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M5R24_RS23205 (4685950) 4685950..4686246 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M5R24_RS23210 (4686474) 4686474..4686746 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M5R24_RS23215 (4686960) 4686960..4690889 - 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4685749..4701837 16088


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T255577 WP_001173343.1 NZ_CP103562:c4685922-4685749 [Escherichia coli O25b:H4-ST131]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT255577 WP_001312331.1 NZ_CP103562:c4686246-4685950 [Escherichia coli O25b:H4-ST131]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References