Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 331625..332425 Replicon chromosome
Accession NZ_CP103562
Organism Escherichia coli O25b:H4-ST131 strain 751

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag M5R24_RS01565 Protein ID WP_000342452.1
Coordinates 331898..332425 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag M5R24_RS01560 Protein ID WP_001277107.1
Coordinates 331625..331891 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5R24_RS01540 (327284) 327284..327952 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
M5R24_RS01545 (327945) 327945..329003 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
M5R24_RS01550 (329248) 329248..330102 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
M5R24_RS01555 (330373) 330373..331476 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
M5R24_RS01560 (331625) 331625..331891 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
M5R24_RS01565 (331898) 331898..332425 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
M5R24_RS01570 (332422) 332422..332805 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
M5R24_RS01575 (333228) 333228..334337 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
M5R24_RS01580 (334385) 334385..335311 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M5R24_RS01585 (335308) 335308..336585 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
M5R24_RS01590 (336582) 336582..337349 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T255557 WP_000342452.1 NZ_CP103562:331898-332425 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT255557 WP_001277107.1 NZ_CP103562:331625-331891 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References