Detailed information of TA system TA025555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3771690..3772364
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TI58
• Locus tag: MCAN_RS17595
• Protein ID: WP_014001683.1
• Coordinates: 3771690..3772118 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: MCAN_RS17600
• Protein ID: WP_003417286.1
• Coordinates: 3772122..3772364 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS17570	3767512..3767913	-	402	WP_003417264.1	cytidine deaminase	-
MCAN_RS17575	3768054..3768488	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
MCAN_RS17580	3768485..3768919	+	435	WP_014001681.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
MCAN_RS17585	3769048..3770820	+	1773	WP_014001682.1	succinate dehydrogenase flavoprotein subunit	-
MCAN_RS17590	3770820..3771611	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
MCAN_RS17595	3771690..3772118	-	429	WP_014001683.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS17600	3772122..3772364	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
MCAN_RS17605	3772487..3773101	-	615	WP_014001684.1	class I SAM-dependent methyltransferase	-
MCAN_RS17610	3773098..3773763	-	666	WP_014001685.1	molybdopterin converting factor subunit 1	-
MCAN_RS17615	3773764..3774297	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
MCAN_RS17620	3774294..3774668	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
MCAN_RS17625	3774765..3775829	-	1065	WP_080603772.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15818.16 Da
• Isoelectric Point: 8.5471

>T25555 WP_014001683.1 NC_015848:c3772118-3771690 [Mycobacterium canettii CIPT 140010059]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQAIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T25555 NC_015848:c3772118-3771690 [Mycobacterium canettii CIPT 140010059]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGCTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCGATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCGAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGGTTTGTCACCTTCGATCAGGCTATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT25555 WP_003417286.1 NC_015848:c3772364-3772122 [Mycobacterium canettii CIPT 140010059]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT25555 NC_015848:c3772364-3772122 [Mycobacterium canettii CIPT 140010059]
ATGCGCACCACGTTATCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: G0TI58

Antitoxin

• AlphaFold DB: G0TI59