Detailed information of TA system TA255534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4191330..4193019
• Replicon: chromosome
• Accession: NZ_CP103559
• Organism: Escherichia coli strain 3059

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: M5T18_RS20490
• Protein ID: WP_000365809.1
• Coordinates: 4191330..4192250 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: M5T18_RS20495
• Protein ID: WP_001550661.1
• Coordinates: 4192237..4193019 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M5T18_RS20475 (4187420)	4187420..4188547	-	1128	WP_001550663.1	MFS transporter	-
M5T18_RS20480 (4188689)	4188689..4189747	-	1059	WP_122987197.1	alpha/beta hydrolase	-
M5T18_RS20485 (4190012)	4190012..4190896	+	885	WP_001119037.1	LysR family transcriptional regulator	-
M5T18_RS20490 (4191330)	4191330..4192250	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M5T18_RS20495 (4192237)	4192237..4193019	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M5T18_RS20500 (4193335)	4193335..4196544	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
M5T18_RS20505 (4196891)	4196891..4197361	+	471	WP_000201185.1	hypothetical protein	-
M5T18_RS20510 (4197358)	4197358..4197460	+	103	Protein_4016	helix-turn-helix domain-containing protein	-
M5T18_RS20515 (4197552)	4197552..4197710	+	159	Protein_4017	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T255534 WP_000365809.1 NZ_CP103559:c4192250-4191330 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT255534 WP_001550661.1 NZ_CP103559:c4193019-4192237 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: V0Z9M0