Detailed information of TA system TA025553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3241773..3242460
• Replicon: chromosome
• Accession: NC_015848
• Organism: Mycobacterium canettii CIPT 140010059

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TFH1
• Locus tag: MCAN_RS15220
• Protein ID: WP_014001473.1
• Coordinates: 3242017..3242460 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: MCAN_RS15215
• Protein ID: WP_003414620.1
• Coordinates: 3241773..3242030 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCAN_RS15195	3237093..3237947	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
MCAN_RS15200	3238003..3239166	-	1164	WP_014001471.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MCAN_RS15205	3239183..3240397	-	1215	WP_014001472.1	zinc metalloprotease Rip	-
MCAN_RS15210	3240405..3241646	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
MCAN_RS15215	3241773..3242030	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
MCAN_RS15220	3242017..3242460	+	444	WP_014001473.1	type II toxin-antitoxin system VapC family toxin	Toxin
MCAN_RS15225	3242540..3243202	+	663	WP_014001474.1	cell surface glycolipoprotein Mpt83	-
MCAN_RS15230	3243298..3243489	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MCAN_RS15235	3243821..3245569	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
MCAN_RS15240	3245665..3246246	+	582	WP_003414644.1	fasciclin domain-containing protein	-
MCAN_RS15245	3246346..3246612	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16625.74 Da
• Isoelectric Point: 6.4890

>T25553 WP_014001473.1 NC_015848:3242017-3242460 [Mycobacterium canettii CIPT 140010059]
MLCVDVNVLVYAHRADLREHTDYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T25553 NC_015848:3242017-3242460 [Mycobacterium canettii CIPT 140010059]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACACGGATTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT25553 WP_003414620.1 NC_015848:3241773-3242030 [Mycobacterium canettii CIPT 140010059]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT25553 NC_015848:3241773-3242030 [Mycobacterium canettii CIPT 140010059]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: G0TFH1

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0