Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3149499..3150283 Replicon chromosome
Accession NZ_CP103559
Organism Escherichia coli strain 3059

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M5T18_RS15630 Protein ID WP_000613626.1
Coordinates 3149789..3150283 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M5T18_RS15625 Protein ID WP_001110447.1
Coordinates 3149499..3149792 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T18_RS15615 (3144649) 3144649..3145608 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M5T18_RS15620 (3146181) 3146181..3149366 + 3186 WP_001550951.1 ribonuclease E -
M5T18_RS15625 (3149499) 3149499..3149792 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M5T18_RS15630 (3149789) 3149789..3150283 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M5T18_RS15635 (3150378) 3150378..3151331 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M5T18_RS15640 (3151343) 3151343..3152986 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
M5T18_RS15645 (3153052) 3153052..3153993 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M5T18_RS15650 (3153993) 3153993..3155090 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T255529 WP_000613626.1 NZ_CP103559:3149789-3150283 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT255529 WP_001110447.1 NZ_CP103559:3149499-3149792 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References